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KMID : 0545119970070010075
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Journal of Microbiology and Biotechnology
1997 Volume.7 No. 1 p.75 ~ p.80
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Biomass Production of Saccharomyces cerevisiae KFCC 10823 and Its Use in Prepararion of Doenjang
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Yoo, Jin Young
Kim, Hyeon Gyu/Kwon, Dong Jin
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Abstract
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An ethanolic fermentation process was developed for preparing Doenjang with high ethanol. Higher and efficient viable cell production of salt-tolerant ethanolic yeast is a prerequisite for the successful commercial-scale process of ethanol production during Doenjang fermentation. Culture conditions of salt-tolerant yeast, S. cerevisiae KFCC 10823, was studied in terms of the effect of several environmental and nutritional factors. Viable cell numbers were the highest in a medium containing the following components per liter of water: soysauce, 300 ㎖; dextrose, 50 g; beef extract, 5 g; yeast extract, 5 g; KH_2PO_4, 5 g; NaCl, 50 g. The optimal culture conditions of S. cerevisiae KFCC 10823 were pH 5.5, 25℃, 200 rpm and 0.5 vvm. Yeast viability during batch fermentation was gradually decreased to a level less than 90% after 35 hours. The maximum cell number was 2.2×10 exp (7) cells/㎖ at the optimal condition. Doenjang prepared with ethanolic yeast was ripened after 45 days at 30℃. This Doenjang contains 470 ㎎% of amino nitrogen and 2.5% ethanol. The shelf-life at 30℃ was theoretically estimated as 444 days.
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